The enhancement of the growth ability and the viability of some probiotic bacteria in media with wild Origanum vulgare L. extract
A Vasile, TL Miron, D Paraschiv, G Bahrim, S Dima
Romanian Biotechnological Letters (2011), 16 (6).
The growth ability and the viability of the Lactobacillus acidophilus and Lactobacillus casei probiotic strains in media Origanum vulgare L., extract rich in phenolic constituents had a good antioxidant capacity in biologically active compounds were evaluated. An extract of Origanum vulgare L. from wild Romanian mountains flora, obtained by pressurized liquid extraction (PLE), was used in order to show the beneficial role of bioactive compounds on the two probiotic strains in culture and in the aggressive conditions of the simulated gastric juice. The results showed that the growth of Lactobacillus acidophilus and Lactobacillus casei was positively influenced by the addition of 20 mg/mL extract of Origanum vulgare L. in MRS broth. After 4 hours of cultivation at 37 °C, in anaerobe conditions the viability of probiotic bacteria in simulated gastric conditions (pH 2.0, time of 0, 30, 60 and 90 min and 50 rpm) in the presence of the same concentration of Origanum vulgare L., the extract was evaluated. The resistance of probiotic bacteria in simulated gastric conditions is enhanced of 1.25 fold, after 30 minutes of incubation, compared with control without added vegetal extract.Article request